Bioaugmentation with Caldibacillus thermoamylovorans improved methane production of thermophilic anaerobic digestion via enhancing hydrolysis and acidification processes
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作者
Xiao, Mengyao; Wang, Na; Xu, Lin; Cai, Dongqing; Zhu, Yanping; Shi, Jiping; Zhang, Siying; Liu, Li
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刊物名称
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
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年、卷、文献号
2025, 5,
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关键词
Xiao, Mengyao; Wang, Na; Xu, Lin; Cai, Dongqing; Zhu, Yanping; Shi, Jiping; Zhang, Siying; Liu, Li
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摘要
Bioaugmentation for enhancing the performance of thermophilic anaerobic digestion (TAD) is attracting more focus. This study aimed to investigate the effect and mechanism of bioaugmentation with Caldibacillus thermoamylovorans QK5 on improving the methane production via TAD using food waste (FW). The results demonstrated that the inoculation of C. thermoamylovorans QK5 could increase the cumulative methane production from 380.33 NmL g-1 VS to 477.56 NmL g-1 VS, with an improvement of 25.57 %. Metagenomic analysis revealed that this effect was achieved by enhancing hydrolysis and acidification stages of TAD. The addition of C. thermoamylovorans QK5 effectively intensified the genes encoding key enzymes involved in the hydrolysis, acidification, and complete oxidation of carbohydrates, amino acids, and fatty acids into CO2, particularly various carbohydrate-active enzymes. Moreover, the C. thermoamylovorans QK5 can settle and reproduce in the TAD system, indicating that it is a reliable and effective strain for bioaugmentation of TAD from FW.
