The biosafety level-2 macromolecular crystallography beamline (BL10U2) at the Shanghai Synchrotron Radiation Facility
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作者
Xu, Qin; Kong, Hua-Ting; Liu, Ke; Zhou, Huan; Zhang, Kun-Hao; Wang, Wei-Wei; Li, Min-Jun; Pan, Qiang-Yan; Wang, Xing-Ya; Wang, Yu-Zhu; Yu, Feng; Gao, Xing-Yu; Wang, Qi-Sheng
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刊物名称
NUCLEAR SCIENCE AND TECHNIQUES
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年、卷、文献号
2023, 34, 2210-3147
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关键词
Xu, Qin; Kong, Hua-Ting; Liu, Ke; Zhou, Huan; Zhang, Kun-Hao; Wang, Wei-Wei; Li, Min-Jun; Pan, Qiang-Yan; Wang, Xing-Ya; Wang, Yu-Zhu; Yu, Feng; Gao, Xing-Yu; Wang, Qi-Sheng
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摘要
BL10U2 is an undulator-based macromolecular crystallography (MX) beamline located at the 3.5-GeV Shanghai Synchrotron Radiation Facility. BL10U2 is specifically designed for conducting routine and biosafety level-2 (BSL-2) MX experiments utilizing high-flux tunable X-rays with energies from 7 to 18 keV, providing a beam spot size of 20 mu m (horizontal) x 10 mu m (vertical) at the sample point. Certification by the Shanghai Pudong Municipal Health Commission confirmed the capability to perform BSL-2 MX experiments. The beamline is currently equipped with an Eiger X 16 M detector and two newly developed in-house high-precision diffractometers that can be switched to perform conventional or in situ crystal diffraction experiments. An automatic sample changer developed in-house allows fast sample exchange in less than 30 s, supporting high-throughput MX experimentation and rapid crystal screening. Data collection from both the diffractometer and detector was controlled by an in-house developed data collection software (Finback) with a user-friendly interface for convenient operation. This study presents a comprehensive overview of the facilities, experimental methods, and performance characteristics of the BL10U2 beamline.
