Functional analysis of acyl-CoA dehydrogenases and their application to C22 steroid production
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作者
Yuan, Chenyang; Li, Yixin; Han, Suwan; He, Beiru; Zhai, Xinghui; Lin, Weichao; Shi, Jiping; Sun, Junsong; Zhang, Baoguo
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刊物名称
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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年、卷、文献号
2023, 107, 1432-0614
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关键词
Yuan, Chenyang; Li, Yixin; Han, Suwan; He, Beiru; Zhai, Xinghui; Lin, Weichao; Shi, Jiping; Sun, Junsong; Zhang, Baoguo
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摘要
Acyl-CoA dehydrogenase (ChsE) is involved in the steroid side-chain degradation process. However, their function in vivo remains unclear. In this study, three ChsE, ChsE1-ChsE2, ChsE3, and ChsE4-ChsE5, were identified in Mycolicibacterium neoaurum, and their functions in vivo are studied and compared with those from Mycobacterium tuberculosis in vitro. By gene knockout, complementation, and the bioconversion of phytosterols, the function of ChsE was elucidated that ChsE4-ChsE5 could utilize C27, C24, and C22 steroids in vivo. ChsE3 could utilize C27 and C24 steroids in vivo. ChsE1-ChsE2 could utilize C27, C24, and C22 steroids in vivo. What is more, the production strain of a C22 steroid, 3-oxo-4,17-pregadiene-20-carboxylic acid methyl ester (PDCE), is constructed with ChsE overexpression. This study improved the understanding of the steroid bioconversion pathway and proposed a method of the production of a new C22 steroid.
