性别 男
职称 正高级工程师四级
电子邮件 haoj@sari.ac.cn
通讯地址 上海市浦东新区海科路99号
个人网页 https://people.ucas.ac.cn/~0017628 ; https://orcid.org/0000-0002-4403-8188
招生专业
化学工程与技术;生物与医药;简历
郝健,博士,研究员,中国科学院大学博士生导师,宁波诺丁汉大学联合博士生导师。
郝健是英国皇家学会牛顿高级学者(Royal Society-Newton Advanced Fellowship)。牛顿高级学者基金重点支持处于中早期职业生涯并取得显著成就的国际学者,基金获得者获得“牛顿高级学者”称号。
郝健主要从事生物化学工程方面的科学研究工作。专注以克雷伯氏菌为细胞工厂,研究该菌生产化学品的能力。开发了克雷伯氏肺炎杆菌生产葡萄糖酸、2-酮基-葡萄糖酸、木糖酸、R-乙偶姻、2R,3R丁二醇、乙二醇、乙醇酸、2-酮基异戊酸、2,3-二羟基异戊酸、2-羟基异戊酸、二羟基丙酮、甘油、缬氨酸、异丁醇、丙醇、1,2-丙二醇等化学品的技术。相关研究工作以通讯作者或第一作者发表研究论文41篇,其中SCI收录30篇。以第一发明人申请国家发明专利27项,其中19项已经授权。
郝健积极开展国际合作与交流,与伦敦大学学院(University College London)、韩国生命工学研究院(Korea Research Institute of Bioscience and Biotechnology)等国际机构建立良好的合作关系。
欢迎生物工程、生物科学、生物化工、生物制药等相关专业优秀学生报考中国科学院大学硕士和博士研究生。
欢迎报考宁波诺丁汉大学-中国科学院上高等研究院联合博士生项目(名额充足)。
研究方向
1. 合成生物学
2. 代谢工程
获奖及荣誉
(1) 牛顿高级学者, 2018,英国皇家学会
(2) 中英优秀青年奖学金, 2007,国家留学基金委
(3) 生物法耦合生产生物柴油和1,3-丙二醇的应用基础研究, 2006,中国石油和化学工业协会科技进步一等奖
承担科研项目情况
[1] 中国科学院战略性先导科技专项(C类),XDC0110202,关键化工材料产品生产菌种创建,2023.9-2026.8,项目负责人,200万元。
[2] 上海市自然科学基金,23ZR1471100,Pdu微室在克雷伯氏菌代谢甘油合成1,3-丙二醇中的功能研究,2023.4-2026.3,项目负责人,20万元。
[3] 中国科学院-韩国国家科技理事会协议项目,184131KYSB20200029,生物法生产异丁醇,2021.1-2022.12,项目负责人,30万元。
[4] 国家重点研发计划,中国和韩国政府间产业技术研发合作项目,2019YFE0196900,生物法生产1,3-丙二醇,2020.12-2022.11,项目负责人,71.5万元。
[5] 上海市自然科学基金,2019.7-2022.6 19ZR1463600,克雷伯氏菌天然异丁醇合成途径的代谢机理研究,项目负责人,20万元。
[6] 英国皇家学会牛顿高级学者基金(Royal Society-Newton Advanced Fellowship grant),NAF\R2\180721,Combined engineering and biological approaches to improve the efficiency of isobutanol production, 2018.11-2021.10, 项目负责人,9.56万英镑。
代表论著
[1] Jiang Weiyan, et al., Inactivation of hydrogenase-3 leads to enhancement of 1,3-propanediol and 2,3-butanediol production by Klebsiella pneumoniae. Enzyme and Microbial Technology. 2024.110438
[2] Wang Qinghui, et al., 2-hydroxyisovalerate production by Klebsiella pneumoniae. Enzyme and Microbial Technology. 2023. 110330
[3] Shu Lin, et al., The pyruvate decarboxylase activity of IpdC is a limitation for isobutanol production by Klebsiella pneumoniae. Biotechnology for Biofuels and Bioproducts 2022. 15:41
[4] Wang Qinghui, et al., Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in L-valine production. World Journal of Microbiology and Biotechnology 2022. 38:81
[5] Shu Lin, et al., The roles of diol dehydratase from pdu operon on glycerol catabolism in Klebsiella pneumoniae. Enzyme and Microbial Technology. 2022. 157: 110021
[6] Sun Shaoqi, et al., 1,2?Propanediol production from glycerol via an endogenous pathway of Klebsiella pneumoniae. Applied Microbiology and Biotechnology. 2021. 105: 9003-9016
[7] Sun Shaoqi, et al., Redirection of the central metabolism of Klebsiella pneumoniae towards dihydroxyacetone production. Microbial Cell Factories. 2021. 20: 123.
[8] Yang Yang, et al.,. Production of 2,3-dihydroxyisovalerate by Enterobacter cloacae. Enzyme and Microbial Technology. 2020 (140): 109650.
[9] Lu Xiyang, et al., Ethylene glycol and glycolic acid production by wild-type Escherichia coli. Biotechnology and Applied Biochemistry. 2021. 68(4): 744-755.
[10] Wang Yike, et al., 2,3-Dihydroxyisovalerate production by Klebsiella pneumoniae. Applied Microbiology and Biotechnology 2020. 104(15): 6601-6613.
[11] Zhang Zhongxi, et al., Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae. Microbial Cell Factories. 2020. 19:89.
[12] Dong Wei, et al., Non-capsulated mutants of a chemical-producing Klebsiella pneumoniae strain. Biotechnology Letter 2018. 40 (4): 1-9.
[13] Gu Jinjie, et al., Isobutanol and 2-ketoisovalerate production by Klebsiella pneumoniae via a native pathway. Metabolic Engineering 2017. 43:71-84.
[14] Zhou Jidong, et al., The role of the pyruvate acetyl-CoA switch in the production of 1,3-propanediol by Klebsiella pneumoniae. Applied Biochemistry and Biotechnology, 2017. 181:1199-1210.
[15] Wang Chenhong, et al., Production of xylonic acid by Klebsiella pneumoniae. Applied Microbiology and Biotechnology, 2016. 100, 23, 10055-10063.
[16] Wang Dexin, et al., Gluconic acid production by gad mutant of Klebsiella pneumoniae World J Microbiol Biotechnol, 2016, 32:132.
[17] Wang Dexin, et al., R-acetoin accumulation and dissimilation in Klebsiella pneumoniae. Journal of Industrial Microbiology & Biotechnology, 2015. 42(8):1105-1115.
[18] Sun Yuehong, et al., Two-stage fermentation for 2-ketogluconic acid production by Klebsiella pneumoniae. Journal of Microbiology and Biotechnology, 2014. 24(6):781-787.
[19] Wei Dong, et al., Role of dihydroxyacetone kinases I and II in the dha regulon of Klebsiella pneumoniae. Journal of Biotechnology, 2014. 177:13–19.
[20] Chen Chuan, et al., Mechanism of 2,3-butanediol stereoisomer formation in Klebsiella pneumoniae. Applied Microbiology and Biotechnology, 2014. 98 (10):4603-4613.